Highly efficient base editing at PCSK9 and normal human embryo development.

Production of Cell Models with Differential Zygosity Using CRISPR Base Editors.

Compact 9dBEs Enable Efficient and Precise Genome Editing in Mammalian Cells and In Vivo

An efficient Cas9d system (Cas9dUltra) is developed through gRNA and protein engineering, and its base editors (9dBEs) further developed through gRNA and protein engineering, enabling efficient and precise genome editing in human cells.

Next-Generation Genome Editing: Overcoming CRISPR-Cas9 Limitations with Prime Editing and PASTE Technology

CRISPR-Cas9 has transformed gene editing; however, it is based on doublestrand DNA breaks, which may result in unintentional mutations and structural alterations, thereby limiting its clinical translation. Instead of DSBs, new precision instruments, including prime editing, can be used to perform accurate search-an...

PS4-8. Efficient DNA-free Genome Editing in Chicken Cells by Delivering mRNA and Protein Form of cas9.

Genome editing in avian systems has largely relied on plasmid-based CRISPR systems and antibiotic selection to achieve successful editing. However, plasmid delivery can result in prolonged nuclease expression and potential unintended DNA integration. In addition, selection-based enrichment may mask the intrinsic geno...

Maternal DNA repair safeguards genome stability during the oocyte-to-embryo transition.

De novo mutations are a major source of genetic variation and disease risk, yet the developmental timing and mechanisms underlying their origin require further investigation. While germ cells have traditionally been considered the primary source of these mutations, increasing evidence suggests that a substantial fracti...

Combinatorial CRISPR/Cas12a excises targeted loci in the mammalian genome at very low efficiency

The CRISPR/Cas12a system has emerged as a highly useful tool for genomic editing due to its ability to process multiple guide RNAs (gRNAs) from a single promoter. In this study, we explored the potential of combinatorial CRISPR/Cas12a to excise targeted loci in the mammalian genome by inducing double-strand breaks (DSB...